Fungal cell biologist

Curriculum Vitae

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EDUCATION
2019-2021
Master degree in molecular and cellular biology
Université Claude Bernard Lyon1 – FRANCE

2019
University exchange
Western University – CANADA

2016-2019
Bachelor degree in genetics and cellular biology
Université Claude Bernard Lyon1 – FRANCE

TEACHING
September 2021 – August 2023
Teaching assistant/Lab instructor
INSA Lyon – FRANCE

September 2019 – June 2021
Undergrade tutor
Université Claude Bernard Lyon1 – FRANCE

STRENGTH
Studious
Ideation
Responsability
Strategic
Input

COMMUNICATIONS
Posters in two international scientific events
Talks in two national scientific events

SCIENTIFIC EXPERIENCE
October 2021 – December 16th 2024
PhD in Molecular and Cellular aspects of biology
Laboratory Microbiology Adaptation and Pathogeny
Team Functional Genomics of Phytopathogenic Fungi
Under the supervision of Christophe BRUEL and Mathias CHOQUER

Titled Of content, space and time: characterization of secretory vesicles in the phytopathogen Botrytis cinerea, the aim of the thesis was to characterize the protein content (Axis 1) and spatial and temporal dynamics (Axis 2) of secretory vesicles in Botrytis cinerea. In Axis 1, we developed protocoles for the enrichment of secretory vesicles that led to the obtention of, to our knowledge, the first proteome of secretory vesicles in a filamentous fungus. Axis 2 led to the development of image and signal analysis pipelines in Python to study spatial and temporal dynamics from confocal time-lapse acquisitions. This study led to the identification of subpopulations of hyphae regarding the dynamics of secretory vesicles accumulation in growing filaments.

January 2021 – June 2021
Internship
Laboratory Microbiology Adaptation and Pathogeny
Team Functional Genomics of Phytopathogenic Fungi

Development of an approach for the measure of susceptibility to parietal stresses in Botrytis cinerea mutants.

January 2020 – February 2020
Intership
Laboratory Microbiology Adaptation and Pathogeny
Team Functional Genomics of Phytopathogenic Fungi

Plasmids production and transformation for the production of Botrytis cinerea mutant strains with impaired secretory vesicles biogenesis.

WET LAB SKILLS
Genetic
      Plasmid design
      Cloning
Bacterial transformation
Fungal transformation
Protein
      Western Blot
      Immunocapture
Microscopy
      Confocal (ZEISS)
      Epifluorescence (LEICA, ECHO)

DRY LAB SKILLS
Coding
      Python
            Image and signal processing
            Automated annotation of proteomic datasets
      FIJI (ImageJ)
            Macro design
            Image pre-processing

LANGUAGES
French
English